木下班
木下班・論文発表
Yong, L.K., and *Kodama, Y.
Dark-induced chloroplast relocation depends on actin filaments in the liverwort Apopellia endiviifolia along with the light- and cold-induced relocations.
Plant Cell Environ., in press (2023) Link
Takagi, H., Hempton, A.K., and *Imaizumi, T.
Photoperiodic flowering in Arabidopsis: Multilayered regulatory mechanisms of CONSTANS and the florigen FLOWERING LOCUS T.
Plant Commun., in press (2023) Link
Ding, M., Zhu, Y., and *Kinoshita, T.
Stomatal properties of Arabidopsis cauline and rice flag leaves and their contributions to seed production and grain yield.
J. Exp. Bot., in press (2023) Link
Kinoshita, S., Suzuki, T., Kiba, T., Sakakibara, H., and *Kinoshita, T.
Photosynthetic-product-dependent activation of plasma membrane H+-ATPase and nitrate uptake in Arabidopsis leaves.
Plant Cell Physiol., 64, 191-203 (2023) Link
Ichikawa, S., Ishikawa, K., Miyakawa, H., and *Kodama, Y.
Live-cell imaging of the chloroplast outer envelope membrane using fluorescent dyes.
Plant Direct, 6, e462 (2022) Link
Blair, E.J., Goralogia, G.S., Lincoln, M.J., Imaizumi, T., and *Nagel, D.H.
Clock-controlled and cold-induced CYCLING DOF FACTOR6 alters growth and development in Arabidopsis.
Front. Plant Sci., 13, 919676 (2022) Link
Soda, M.N., Hayashi, Y., Takahashi, K., and *Kinoshita, T.
Tryptophan synthase ß subunit 1 affects stomatal phenotypes in Arabidopsis thaliana.
Front. Plant Sci., 13, 1011360 (2022) Link
*Ando, E., Kollist, H., Fukatsu, K., *Kinoshita, T., and Terashima, I.
Elevated CO2 induces rapid dephosphorylation of plasma membrane H+-ATPase in guard cells.
New Phytol., 236, 2061–2074 (2022) Linkプレスリリース
*Friml, J., Gallei, M., Gelová, Z., Johnson, A., Mazur, E., Monzer, A., Rodriguez, L., Roosjen, M., Verstraeten, I., Živanović, B.D., Zou, M., Fiedler, L., Giannini, C., Grones, P., Hrtyan, M., Kaufmann, W.A., Kuhn, A., Narasimhan, M., Randuch, M., Rýdza, N., Takahashi, K., Tan, S., Teplova, A., Kinoshita, T., Weijers, D., and Rakusová, H.
ABP1–TMK auxin perception for global phosphorylation and auxin canalization.
Nature, 609, 575–581 (2022) Link
Ueda, A., Aihara, Y., Sato, S., Kano, K., Mishiro-Sato, E., Kitano, H., Sato, A., *Fujimoto, K.J., Yanai, T., *Amaike, K., *Kinoshita, T., and *Itami, K.
Discovery of 2,6-dihalopurines as stomata opening inhibitors: implication of an LRX-mediated H+-ATPase phosphorylation pathway.
ACS Chem. Biol., 18, 347-355 (2023) Link
*Inoue, S.I, Hayashi, M., Huang, S., Yokosho, K., Gotoh, E., Ikematsu, S., Okumura, M., Suzuki, T., Kamura, T., *Kinoshita, T., and *Ma, J.F.
A tonoplast-localized magnesium transporter is crucial for stomatal opening in Arabidopsis under high Mg2+ conditions.
New Phytol., 236, 864-877 (2022) Linkプレスリリース
*Kinoshita S.N., and *Kinoshita T.
A win-win scenario for photosynthesis and the plasma membrane H+ pump.
Front. Plant Sci., 13, 982485 (2022) Link
Noguchi, M., and *Kodama, Y.
Temperature sensing in plants: On the dawn of molecular thermosensor research.
Plant Cell Physiol., 63, 737-743 (2022) Link
Ishikawa, K., Konno, R., Hirano, S., Fujii, Y., Fujiwara, M., Fukao, Y, and *Kodama, Y.
The endoplasmic reticulum membrane-bending protein RETICULON facilitates chloroplast relocation movement in Marchantia polymorpha.
Plant J., 111, 205-216 (2022) Link
Hirano, S., Sasaki, K., Osaki, Y., Tahara, K., Takahashi, H., Takemiya, A., and *Kodama, Y.
The localization of phototropin to the plasma membrane defines a cold-sensing compartment in Marchantia polymorpha.
PNAS Nexus, 1, pgac030 (2022) Link
Chonprakun, T., Horii, Y., Mori, M., Fujita, S., Ohtani, M., Tsuchiya, K., Kodama, Y., *Odahara, M., and *Numata K.
Non-transgenic gene modulation via spray delivery of nucleic acid/peptide complexes into plant nuclei and chloroplasts.
ACS Nano, 16, 3506–3521 (2022) Linkプレスリリース
Akiyama, M., Sugimoto, H., Inoue, S., Takahashi, Y., Hayashi, M., Hayashi, Y., Mizutani, M., Ogawa, T., Kinoshita, D., Ando, E., Park, M., Gray, W.M., and *Kinoshita, T.
Type 2C protein phosphatase clade D family members dephosphorylate guard cell plasma membrane H+-ATPase.
Plant Physiol., 188, 2228–2240 (2022) Link
Hayashi, Y., *Takahashi, Y., Fukatsu, K., Tada, Y., Takahashi, K., Kuwata, K., Suzuki, T., and *Kinoshita, T.
Identification of abscisic acid-dependent phosphorylated basic helix-loop-helix transcription factors in guard cells of Vicia faba by mass spectrometry.
Front. Plant Sci., 12, 735271 (2021) Link
Ren, Z., Suolang, B., Fujiwara, T., Yang, D., Saijo, Y., *Kinoshita, T., and *Wang, Y.
Promotion and upregulation of a plasma membrane proton-ATPase strategy: principles and applications.
Front. Plant Sci., 12, 749337 (2021) Link
Lin, W., Zhou, X., Tang, W., Takahashi, K., Pan, X., Dai, J., Ren, H., Zhu, X., Pan, S., Zheng, H., Gray, W.M., Xu, T., and Kinoshita, T., and *Yang, Z.
TMK-based cell surface auxin signaling activates cell wall acidification in Arabidopsis.
Nature, 599, 278-282 (2021) Link
Li, L., Verstraeten, I., Roosjen, M., Takahashi, K., Rodriguez, L., Merrin, J., Chen, J., Shabala, L., Smet, W., Hong, R.H., Vanneste, S., Shabala, S., De Rybel, B., Weijers, D., Kinoshita, T., Gray, W.M., and *Friml, J.
Cell surface and intracellular auxin signalling for H+ fluxes in root growth.
Nature, 599, 273-277 (2021) Link
*Toh, S., Takata, N., Ando, E., Toda, Y., Wang, Y., Hayashi, Y., Mitsuda, N., Nagano, S., Taniguchi, T., and *Kinoshita, T.
Overexpression of plasma membrane H+-ATPase in guard cells enhances light-induced stomatal opening, photosynthesis, and plant growth in hybrid aspen.
Front. Plant Sci., 12, 766037 (2021) Link
Ye, W., Koya, S., Hayashi, Y., Jiang, H., Oishi, T., Kato, K., Fukatsu, K., and *Kinoshita, T.
Identification of genes preferentially expressed in stomatal guard cells of Arabidopsis thaliana and involvement of the aluminum-activated malate transporter 6 vacuolar malate channel in stomatal opening.
Front. Plant Sci., 12, 744991 (2021) Link
Ding, M., Zhang, M., Zeng, H., Hayashi, Y., Zhu, Y., and *Kinoshita, T.
Molecular basis of plasma membrane H+-ATPase function and potential application in the agricultural production.
Plant Physiol. Biochem., 168, 10–16 (2021) Link
Wang, T., Ye, W., Wang, Y., Zhang, M., Aihara, Y., and *Kinoshita, T.
Protease inhibitor-dependent inhibition of light-induced stomatal opening.
Front. Plant Sci., 12, 735328 (2021) Link
*Toda, Y., *Tameshige, T., Tomiyama, M., Kinoshita, T., and Shimizu, K.K.
An affordable image-analysis platform to accelerate stomatal phenotyping during microscopic observation.
Front. Plant Sci., 12, 715309 (2021) Link
*Kinmonth-Schultz, H., Lewandowska-Sabat, A., Imaizumi, T., Ward, J.K., Rognli, O.A., and *Fjellheim, S.
Flowering times of wild Arabidopsis accessions from across Norway correlate with expression levels of FT, CO, and FLC genes.
Front. Plant Sci., 12, 747740 (2021) Link
Kubota, A., Song, Y.H., and *Imaizumi T.
Simple nuclei preparation and co-immunoprecipitation procedures for studying protein abundance and interactions in plant circadian time courses.
Methods Mol. Biol., 2398, 121-134 (2022) Link
Kato, S., Takahashi, Y., Fujii, Y., Sasaki, K., Hirano, S., Okajima, K., and *Kodama, Y.
The photo-thermochemical properties and functions of Marchantia phototropin encoded by an unduplicated gene in land plant evolution.
J. Photochem. Photobiol. B., 224, 112305 (2021) Link
Takahashi, H., and *Kodama, Y.
Culture-based preservation of Marchantia polymorpha gemmalings and thalli without encapsulation, drying, or freezing.
Plant Biotechnol., 38, 449-452 (2021) Link
Yokawa, K., and *Kodama, Y.
A green light-excitable FRET system for monitoring intracellular calcium levels in plant cells.
Plant Signal. Behav., 1963104 (2021) Link
Kamiyama, Y., Hirotani, M., Ishikawa, S., Minegishi, F., Katagiri, S., Rogan, C., Takahashi, F., Nomoto, M., Ishikawa, K., Kodama, Y., Tada, Y., Takezawa, D., Anderson, J., Peck, S., Shinozaki, K., and *Umezawa, T.
Arabidopsis group C Raf-like protein kinases negatively regulate abscisic acid signaling and are direct substrates of SnRK2.
Proc. Natl. Acad. Sci. USA, 118, e2100073118 (2021) Linkプレスリリース
Yong, L.K., Tsuboyama,. S, Kitamura, R., Kurokura, T., Suzuki, T., and *Kodama, Y.
Chloroplast relocation movement in the liverwort Apopellia endiviifolia.
Physiol. Plant., 173, 775-787 (2021) Link
Oikawa, K., Imai ,T., *Kodama K., and *Numata, K.
Effects of mitochondria-selective fluorescent probes on mitochondrial movement in Arabidopsis mesophyll cells evaluated by using the quantification..
Plant Biotechnol., 38, 257-262 (2021) Link
Oikawa, K., Imai, T., Thagun, C., Toyooka, K., Yoshizumi, T., Ishikawa, K., *Kodama, K., and *Numata, K.
Mitochondrial movement during its association with chloroplasts in Arabidopsis thaliana.
Commun. Biol., 4, 292 (2021) Linkプレスリリース
Sanagi, M., Aoyama, S., Kubo, A., Lu, Y., Sato, Y., Ito, S., Abe, M., Mitsuda, N., Ohme-Takagi, M., Kiba, T., Nakagami, H., Rolland, F., Yamaguchi, J., *Imaizumi, T., and *Sato, T.
Low nitrogen conditions accelerate flowering by modulating the phosphorylation state of FLOWERING BHLH 4 in Arabidopsis.
Proc. Natl. Acad. Sci. USA, 118, e2022942118 (2021) Linkプレスリリース
土壌中の窒素量が開花時期に影響を及ぼすという観察は農作物でも見受けられますがそのメカニズムは殆ど分かっていませんでした。本研究では、窒素量に応じてリン酸化の程度が顕著に異なる転写因子が開花制御に重要な因子であるFBH4であることを発見しました。さらに培養条件中の窒素量によりFBH4タンパク質のリン酸化修飾変化が起こり、タンパク質の細胞内局在を変化させることによって転写因子の活性を制御していることが示唆されました。その上FBH4タンパク質はSnRK1リン酸化酵素によりリン酸化される事、また窒素量に応じてSnRK1の活性が変わる事を見出しました。これらのメカニズムを介して窒素量の変化が開花時期を調節する事を明らかにしました。
*Kinoshita, T., Toh, S., and *Torii, K.U.
Chemical control of stomatal function and development.
Curr. Opin. Plant Biol., 60, 102010 (2021) Link
Zhang, M., Wang, Y., Chen, X., Xu, F., Ding, M., Ye, W., Kawai, Y., Toda, Y., Hayashi, Y., Suzuki, T., Zeng, H., Xiao, L., Xiao, X., Xu, J., Guo, S., Yan, F., Shen, Q., Xu, G., *Kinoshita, T., and *Zhu, Y.
Plasma membrane H+-ATPase overexpression increases rice yield via simultaneous enhancement of nutrient uptake and photosynthesis.
Nature Commun., 12, 735 (2021) Link, プレスリリース
植物は、根から窒素などの養分を吸収すると同時に、葉の気孔を開き、CO2を取り込んで光合成を行い、成長しています。本研究では、イネの養分吸収と気孔開口について解析を行い、細胞膜プロトンポンプと呼ばれる酵素が共通して重要な役割を果たすことが明らかとなりました。そこで、プロトンポンプ過剰発現イネの詳細な解析を行ったところ、野生株と比べ、根での養分吸収、気孔開口、光合成活性が20％以上増加し、隔離水田での栽培試験において収量が30%以上増加することが明らかとなりました。さらに過剰発現イネでは窒素の施肥量を半分に減らしても、通常より収量が多いことを見出しました。本研究の成果は、今後、食糧増産や環境問題に大きく関わるCO2や肥料の削減に貢献することが期待されます。
Pudasaini, A., Green, R., Song, Y.H., Blumenfeld, A., Karki, N., Imaizumi, T., and *Zoltowski, B.D.
Steric and electronic interactions at Gln154 in ZEITLUPE induce reorganization of the LOV domain dimer interface.
Biochemistry, 60, 95-103 (2021) Link